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Fig. 1 | Clinical Proteomics

Fig. 1

From: Development of an ID-LC–MS/MS method using targeted proteomics for quantifying cardiac troponin I in human serum

Fig. 1

A diagrammatic illustrating the step-by-step of the analytical workflow for the SI-traceable quantification of cTnI in human serum. Step 1: the calibration standards and QC materials were prepared using cTnI-free serum by incorporating the NIST SRM 2921 and SIL-cTnI. Step 2: patient serum samples were prepared using cTnI positive serum samples by incorporating SIL-cTnI. Step 3: all samples were subjected immunoaffinity enrichment, followed by LC–MS/MS analysis. Step 4: calibration curves were established for the TLLLQIAK and NITEIADLTQK peptides to determine the concentration of cTnI. Step 5: to determine the uncertainty, all sources of uncertainty from steps 1 to 4 were combined

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Clinical Proteomics

ISSN: 1559-0275