Development of an ID-LC–MS/MS method using targeted proteomics for quantifying cardiac troponin I in human serum

Table 2 PRM transitions, amino acid sequence and retention time for the detection of the peptides

Proteotypic peptides	Retention time (min)	Precursor ions (m/z)	Collision energy (eV)	Product ions (m/z)
TLLLQIAK	13.89	450.30	20	572.377
TLLLQIAK	13.89	450.30	20	685.460
TLLLQIAK (U-N15)	13.89	455.28	20	579.356
TLLLQIAK (U-N15)	13.89	455.28	20	693.437
NITEIADLTQK	11.28	623.35	20	675.367
NITEIADLTQK	11.28	623.35	20	1018.542
NITEIADLTQK (U-N15)	11.28	630.31	20	683.311
NITEIADLTQK (U-N15)	11.28	630.31	20	1029.506
DLPSPIER	9.73	463.750	25	514.298
DLPSPIER	9.73	463.750	25	698.383
DLPSPIER (13C6, 15N4)	9.73	468.755	25	524.305
DLPSPIER (13C6, 15N4)	9.73	468.755	25	708.390

ISSN: 1559-0275