Fig. 2

Differentially expressed proteins. The cutoff range of protein identification is as follows: protein confidence interval > 95.0%, peptide N ≥ at least two peptides, 1% < decoy FDR. Through t-test, p ≤ 0.05, and cv < 20%, total 476 proteins were finally listed as differentially expressed proteins. Volcano plot (A): For the analysis of differentially expressed proteins and statistical analysis, Perseus (version 1.5.8.2) and R were used, where the cutoff range for significant fold change (FC) and T-test p-values were set as ± 2.0 and 0.05, respectively. Heat map (B): 2D–hierarchical clustering analysis exploring the difference in protein expression between Red and Green Pashmina fiber. Each row in the map represents a differentially expressed proteins, and each column represents the condition used. Log₂ (DEP) value was used for constructing the heat-map