Table 1 Histone H3 peptides analyzed in the present study (the amino acid, on which the SIL was introduced, is depicted bold and underlined)
Peptide name | Calculated monoisotopic molecular mass | Amino acid sequence | Precursor ion m/z | Precursor ion charge state | Product ion m/z | Product ion type | Product ion charge state | MRM transition m/z | MRM transition name |
|---|---|---|---|---|---|---|---|---|---|
K9[Poy]K14[Ac] | 999.5587 | K[Poy]STGGK[1Ac]APR | 500.3 |  + 2 | 728.4 | y7 |  + 1 | 500.3/728.4 | MRM1 |
500.3 |  + 2 | 815.4 | y8 |  + 1 | 500.3/815.4 | MRM2 | |||
500.3 |  + 2 | 627.6 | y6 |  + 1 | 500.3/627.6 | MRM3 | |||
K9[Poy]K14[Ac]-heavy | 1009.5669 | K[Poy]STGGK[1Ac]APR | 505.3 |  + 2 | 738.4 | y7 |  + 1 | 505.3/738.4 | MRM4 |
505.3 |  + 2 | 825.4 | y8 |  + 1 | 505.3/825.4 | MRM5 | |||
505.3 |  + 2 | 637.6 | y6 |  + 1 | 505.3/637.6 | MRM6 | |||
K9[Poy]K14[Poy] | 1013.5744 | K[Poy]STGGK[Poy]APR | 507.4 |  + 2 | 742.3 | y7 |  + 1 | 507.4/742.3 | MRM7 |
507.4 |  + 2 | 829.3 | y8 |  + 1 | 507.4/829.3 | MRM8 | |||
507.4 |  + 2 | 641.5 | y6 |  + 1 | 507.4/641.5 | MRM9 | |||
K9[Poy]K14[Poy]-heavy | 1023.5826 | K[Poy]STGGK[Poy]APR | 512.3 |  + 2 | 752.4 | y7 |  + 1 | 512.3/752.4 | MRM10 |
512.3 |  + 2 | 839.4 | y8 |  + 1 | 512.3/839.4 | MRM11 | |||
512.3 |  + 2 | 651.4 | y6 |  + 1 | 512.3/651.4 | MRM12 |