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Fig. 1 | Clinical Proteomics

Fig. 1

From: Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions

Fig. 1

(A) Workflow for in-gel and in-solution methods of sample preparation. (B) Comparing accuracy of copper- and Coomassie-based assays for correctly measuring known quantities of protein in water and UW-MPS. Predicted and measured concentrations from 5 experiments were normalised using average and standard deviation, and percentage error calculated (see methods). (C) protein concentrations of all kidney perfusate samples that underwent Coomassie-based protein estimation assay (n = 24). (D&E) Average numbers of proteins identified in the kidney (n = 8) and liver (n = 6) perfusate samples that underwent in-solution digestion and LC-MS/MS analysis, by collection timepoint

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Clinical Proteomics

ISSN: 1559-0275