Table 2 Transitions monitored for the quantitation of apo(a) in both the RMP and the DCM
Peptide | SIL (y/n) | Precursor (m/z) | Quantifying fragment (m/z) | Qualifying fragment 1 (m/z) | Qualifying fragment 2 (m/z) |
|---|---|---|---|---|---|
IFCC-endorsed Reference Measurement Procedure* | |||||
GISSTTVTGR | 489.8 | 808.4 (y8) | 533.3 (y5) | 634.4 (y6) | |
GISSTTVTG[R-U10] | Y | 494.8 | 818.4 | 543.3 | 644.4 |
LFLEPTQADIALLK | 786.5 | 1069.6 (y10) | 261.2 (b2) | 1198.6 (y11) | |
LFLEPTQADIALL[K + U8] | Y | 790.5 | 1077.6 | 261.2 | 1206.6 |
TPENYPNAGLTR | 666.8 | 728.4 (y7) | 199.1 (b2) | 891.5 (y8) | |
TPENYPNAGLT[R + U10] | Y | 671.8 | 738.4 | 199.1 | 901.5 |
Designated Comparison Method | |||||
GISSTTVTGR | 489.8 | 808.4 (y8) | 634.4 (y6) | 533.3 (y5) | |
GISSTTVTG[R-U10] | Y | 494.8 | 818.4 | 644.4 | 543.3 |
LFLEPTQADIALLK | 786.5 | 1069.6 (y10) | 1198.6 (y11) | 374.2 (b2) | |
LFLEPTQADIALL[K + U8] | Y | 790.5 | 1077.6 | 1206.6 | 374.2 |