Quantification of putative ovarian cancer serum protein biomarkers using a multiplexed targeted mass spectrometry assay

Table 4 Concentration of 3 target proteins in 69 patients’ serum samples

Protein concentration calculated from concentration of each peptide corresponding to the protein
UniProt accession #	Peptide sequence	MW (Da)	Peptide concentration in Patients' sera (pmol mL⁻¹; mean ± SD)				Protein concentration in Patients' sera (ng mL⁻¹; mean ± SD)
UniProt accession #	Peptide sequence	MW (Da)	Non-cancerous	Benign	Early-stage	Late-stage	Non-cancerous	Benign	Early-stage	Late-stage
sp\|P18065\|IBP2	LEGEACGVYTPR	34814	1.38 ± 0.64	1.32 ± 0.68	1.47 ± 1.05	2.37 ± 1.45	48.10 ± 22.35	46.13 ± 23.50	51.13 ± 36.65	82.44 ± 50.36
sp\|P18065\|IBP2	LIQGAPTIR	34814	0.48 ± 0.28	0.48 ± 0.28	0.63 ± 0.46	0.99 ± 0.58	16.60 ± 9.83	16.60 ± 9.83	21.97 ± 16.09	34.45 ± 20.22
sp\|P04278\|SHBG	QAEISASAPTSLR	43779	8.77 ± 6.04	8.73 ± 4.61	8.56 ± 2.95	10.62 ± 2.95	383.94 ± 264.34	382.03 ± 201.61	374.65 ± 129.13	464.75 ± 129.19
sp\|P04278\|SHBG	LDVDQALNR	43779	0.91 ± 0.49	0.54 ± 0.28	0.62 ± 0.31	0.73 ± 0.23	39.72 ± 21.30	23.85 ± 12.31	27.23 ± 13.42	32.13 ± 10.29
sp\|P01033\|TIMP1	GFQALGDAADIR	23171	0.69 ± 0.10	0.78 ± 0.28	0.83 ± 0.23	1.07 ± 0.43	16.09 ± 2.25	17.98 ± 6.39	19.18 ± 5.39	24.88 ± 9.86
sp\|P01033\|TIMP1	SEEFLIAGK	23171	1.32 ± 0.14	1.95 ± 1.60	1.69 ± 0.50	1.71 ± 0.60	26.68 ± 10.54	45.20 ± 37.15	39.20 ± 11.56	39.56 ± 13.92

Protein concentration calculated from mean concentration of two peptides corresponding to the protein
UniProt accession #	Peptide sequence	MW (Da)	Peptide concentration in Patients' sera (pmol mL⁻¹; mean ± SD)				Protein concentration in Patients' sera (ng mL⁻¹; mean ± SD)
UniProt accession #	Peptide sequence	MW (Da)	Non-cancerous	Benign	Early-stage	Late-stage	Non-cancerous	Benign	Early-stage	Late-stage
sp\|P18065\|IBP2	LEGEACGVYTPR	34814	0.77 ± 0.51*	0.77 ± 0.40*	1.06 ± 0.73*	1.67 ± 1.00*	26.82 ± 17.82	26.93 ± 14.09	36.83 ± 25.52	58.98 ± 34.67
sp\|P18065\|IBP2	LIQGAPTIR	34814	0.77 ± 0.51*	0.77 ± 0.40*	1.06 ± 0.73*	1.67 ± 1.00*	26.82 ± 17.82	26.93 ± 14.09	36.83 ± 25.52	58.98 ± 34.67
sp\|P04278\|SHBG	QAEISASAPTSLR	43779	5.22 ± 3.32*	6.31 ± 4.94*	5.80 ± 2.57*	6.17 ± 1.80*	228.58 ± 145.39	276.05 ± 216.09	254.03 ± 112.43	270.21 ± 78.60
sp\|P04278\|SHBG	LDVDQALNR	43779	5.22 ± 3.32*	6.31 ± 4.94*	5.80 ± 2.57*	6.17 ± 1.80*	228.58 ± 145.39	276.05 ± 216.09	254.03 ± 112.43	270.21 ± 78.60
sp\|P01033\|TIMP1	GFQALGDAADIR	23171	0.84 ± 0.21*	1.48 ± 1.49*	1.17 ± 0.38*	1.33 ± 0.43*	19.46 ± 4.88	34.28 ± 34.59	27.13 ± 8.82	30.89 ± 10.02
sp\|P01033\|TIMP1	SEEFLIAGK	23171	0.84 ± 0.21*	1.48 ± 1.49*	1.17 ± 0.38*	1.33 ± 0.43*	19.46 ± 4.88	34.28 ± 34.59	27.13 ± 8.82	30.89 ± 10.02

^*represents the mean concentration of two peptides corresponding to each target protein

ISSN: 1559-0275