Absolute quantitation of human wild-type DNAI1 protein in lung tissue using a nanoLC-PRM-MS-based targeted proteomics approach coupled with immunoprecipitation

Table 1 IP-MS analysis for wild-type human lung

Sample	Lot #	Calculated concentration (pg/mg tissue)*
Human lung 01	MHU-P-051915	27.1
Human lung 02	MHU-P-082719	4.37
Human lung 03	MHU-P-082919	14.4
Human lung 04	P1245-Tn8	14.3
Human lung 05	P1060-Tn8	5.82
Human lung 06	P1079-Tn8	9.24
Human lung 07	P1140-Tn10	4.40
Human lung 08	P1053-Tn7	44.7
Human lung 09	K30-Tn7	7.15
Human lung 10	R64-Tn8	12.4
Human lung 11	P1096-Tn7	5.03
Human lung 12	P1574-Tn10	45.6
Human lung 13	P1034-Tn9	19.6
Human lung 14	P1113-Tn10	31.9

^*Calculated concentration (pg/mg tissue) of hDNAI1 was determined based on the standard curve for the peptide AHIFDLAINK from the hDNAI1 protein in tissue matrices

ISSN: 1559-0275